Cationic Amphiphilic Macromolecules (CAMs)-Lipid Complexes for Intracellular siRNA Delivery

Cationic amphiphilic macromolecules (CAMs) were non-covalently blended with lipids (DOPE and DOTAP) to serve as a siRNA delivery vehicle. By varying weight ratio of CAM to lipid, catonic complexes with different compositions were obtained and their properties were evaluated. Certain CAM-lipid (9N-lipid) with weight ratio of 1:10 was found to have significantly higher transfection efficiency than Lipofectamine. The intracellular trafficking was also applied to elucidate that siRNA escaped endosome at 24 hrs after transfection.