Influence of tableting on the conformational and thermal stability of a model protein drug

Category

Processing Technology/Manufacturability

Year

2013

Authors

M. Klukkert, A. Sakmann, T. Rades, , and C.S. Leopold

Institutions

University of Hamburg, Bundesstraße 45, 20146 Hamburg, Germany University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark University of Otago, 18 Frederik Street, 9050 Dunedin, New Zealand

Summary

The influence of tableting on the secondary structure and thermal stability of trypsin was investigated. Structural changes were analyzed by aqueous-state FTIR at isothermal conditions and with temperature scans by chemometrics and Boltzmann fitting. It could be shown that tableting alters the secondary structure of trypsin but only subtlety influence its thermal stability.